Hillert, Lena 1955- [WorldCat Identities]

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Medarbetare Patient Vårdgivare Vårdhygien Regional laboratoriemedicin Analyslistor, provtagningsanvisningar Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). γH2AX, the phosphorylated form of H2AX, is involved in the steps leading to chromatin decondensation after DNA double-strand breaks. γH2AX does not, itself, cause chromatin decondensation, but within 30 seconds of ionizing radiation, RNF8 protein can be detected in association with γH2AX.

Gamma h2ax

In addition 31 Dec 2012 Formation of γ-H2AX in response to DNA double stranded breaks (DSBs) provides the basis for a sensitive assay of DNA damage in human Rabbit polyclonal gamma H2A.X (phospho S139) antibody. Validated in WB, IHC , ICC/IF and tested in Mouse, Human. Cited in 178 publication(s). 28 Sep 2018 Quantification of H2AX levels by immunoblotting was based on the Use of the gamma-H2AX assay to monitor DNA damage and repair in How phosphorylated histone H2AX, known as γ-H2AX, functions in the cellular response to DNA double-strand breaks is the subject of intensive investigation. In experiments involving the use of “laser scissors” to introduce breaks into living cells, γ-H2AX foci localized specifically with the laser path through the cell 21 Jun 2013 Comparison of two methods for measuring γ-H2AX nuclear fluorescence as a marker of DNA damage in cultured human cells: applications for 2 Jun 2020 Simultaneous quantitation of γ-H2AX and p-H3 in exposed cells may monitor the toxicity of genotoxic chemicals and to some extent reflect the γ‐H2AX foci form rapidly at the sites of DNA damage and facilitate the anchoring of signalling and repair proteins, including the MRN complex (NBS1/MRE11/ However, although γ-H2AX appears to be a principal player in the DNA damage response and necessary for the initial rapid phase of DSB repair, mice lacking This modification, called gamma-H2AX (phospho-Ser139), triggers cell cycle arrest and DNA damage response repair, which makes this assay particularly The histone variant H2AX is rapidly phosphorylated at the sites of DNA double- strand breaks (DSBs). This phosphorylated H2AX (γ-H2AX) is involved in the Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified proc … Gamma H2AX (γH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage. As a result of DSBs, H2AX becomes phosphorylated at serine-139 (p Ser139).

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gamma-H2AX (gH2AX) is the phosphorylated form of histone H2AX and can function as a sensitive marker for double-strand breaks (DSBs), which signifies genomic instability and can potentially contribute to cancer initiation and progression. In this way, monitoring the formation of gH2AX can potentially be a sensitive means of cancer Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes.

Annabrita Schoonenberg — Helsingfors universitet

2005 Dec 9;20(5):801-9. Epub 2005 Nov 28. PMID 16310392 : Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. Ionizing Radiation-dependent {gamma}-H2AX Focus Formation Requires Ataxia Telangiectasia Mutated and Ataxia Telangiectasia Mutated and Rad3-related. MOLECULAR BIOLOGY OF THE CELL AT1G08880 AT1G54690 AT3G48190 Anti-phospho-H2A.X (Ser139) Antibody Upstate®, from rabbit; Synonym: H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac; find Sigma-Aldrich-07-164 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Kinner A, Wu W, Staudt C, Iliakis G (2008) Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 36:5678–5694 PubMed CrossRef Google Scholar 2021-02-25 · p-Histone H2A.X Antibody (Ser 139) is a mouse monoclonal IgG 1, cited in 38 publications, provided at 100 µg/ml; raised against a recombinant protein corresponding to the Ser 139 phosphorylated region of Histone H2A.X of human origin gamma-H2AX Antibody.

These results unveil a new interplay between plant H2AX and E2F transcriptional activators during the DNA double-strand breaks response. Gamma-H2AX can also be measured by immunohistochemistry. By counting gamma-H2AX- foci, it is possible to measure very low levels of DNA damage that are inflicted during routine X-ray examinations . A comparison of our flow-cytometry method with published data indicates that foci counting can detect close to ten times lower doses of IR .
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Therefore, the analysis of H2AX phosphorylated (γH2AX) may be possibly used as biomarker of genotoxicity and genomic instability with a number of applications in human epidemiology. However, the lack of an experimental standard leads to a wide heterogeneity in the results obtained and their interpretation, affecting the reliability of the assay. Target Information Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. Abstract The phosphorylation of histone H2AX in Serine 139 (gamma‐H2AX) marks regions of DNA double strand breaks and contributes to the recruitment of DNA repair factors to the site of DNA damage.

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Cited in 269 publication(s). Independently reviewed in 35 review(s). PDF | On Jan 1, 2008, I.M. Rakiman and others published γ-H2AX assay: a technique to quantify DNA double strand breaks | Find, read and cite all the research you need on ResearchGate Unconjugated Whole IgG Rabbit anti-gamma-H2AX Antibody, Affinity Purified suitable for WB, IHC, ICC-IF applications. Visit Bethyl.com for all your antibody needs.

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gamma-H2AX (gH2AX) is the phosphorylated form of histone H2AX and can function as a sensitive marker for double-strand breaks (DSBs), which signifies genomic instability and can potentially contribute to cancer initiation and progression. In this way, monitoring the formation of gH2AX can potentially be a sensitive means of cancer Keywords: Gamma-H2AX, High-content screening, Image processing, Hough transform, dosimetry 1. INTRODUCTION The study of damage to cellular DNA is essential for the understanding of cell apoptosis and mutation that may lead to serious disease including cancer.

HT γ-H2AX Pharmacodynamic Assay. 86 products Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. [pSer¹³⁹]Histone H2AX monoclonal antibody (. Jun 2, 2020 Simultaneous quantitation of γ-H2AX and p-H3 in exposed cells may monitor the toxicity of genotoxic chemicals and to some extent reflect the This modification, called gamma-H2AX (phospho-Ser139), triggers cell cycle arrest and DNA damage response repair, which makes this assay particularly Phosphorylation of Ser-140 (known as gamma-H2AX) is critical during DNA double-strand break signaling.